A novel egg-shell membrane based hybrid nanofibrous scaffold for cutaneous tissue engineering.

ABSTRACT:

Background

The main issue in cutaneous regeneration is to develop engineered scaffolds based on natural extracellular matrix to promote dynamics of skin progenitor cells and accelerate differentiation into mature keratinocytes.

Methods

In this study, nanofibrous scaffolds composed of a blend poly (ɛ-caprolactone) (PCL), silk fibroin (SF), soluble eggshell membrane (SESM), and Aloe vera (AV) gel were developed by electrospinning method and human basal cells were used to examine differentiation capacity toward keratinocyte-like cells. For this propose, cells were allocated to four distinct groups; control, PCL/SF, PCL/SF/SESM, and PCL/SF/SESM/AV. In all groups, cells were incubated with differentiation medium. Morphology, composition, hydrophilicity and mechanical featu

SUBMITTER: Mohammadzadeh L 

PROVIDER: S-EPMC6815433 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature