Selection of appropriate reference genes for RT-qPCR analysis under abiotic stress and hormone treatment in celery.

ABSTRACT: Celery is one of the most important vegetable crop and its yield and quality is influenced by many environmental factors. Researches on gene expression not only help to unravel the molecular regulatory mechanism but also identify the key genes in the biological response. RT-qPCR is a commonly used technology to quantify the gene expression. Selecting an appropriate reference gene is an effective approach to improve the accuracy of RT-qPCR assay. To our knowledge, the evaluation of reference genes under different treatments in celery has not been reported yet. In this study, the expression stabilities of eight candidate reference genes (ACTIN, eIF-4α , GAPDH, TBP, TUB-A, UBC, TUB-B, and EF-1α ) under abiotic stresses (hea

SUBMITTER: Feng K 

PROVIDER: S-EPMC6815649 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature