Salivary Metabolomics of Total Body Irradiated Nonhuman Primates Reveals Long-Term Normal Tissue Responses to Radiation.

ABSTRACT:

Purpose

To identify metabolomic biomarkers of acute radiation exposure in saliva that show time-dependent changes.

Methods and materials

Nonhuman primates were exposed to 4 Gy of total body irradiation with γ-rays. Saliva was collected from 7 animals twice before and at days 1, 3, 5, 7, 15, 21, 28, and 60 after irradiation. Profiling was conducted with liquid chromatography time-of-flight mass spectrometry. Multivariate data analysis and potential biomarker identification was conducted through random Forests and the software MetaboAnalyst. Candidate biomarkers were validated through tandem mass spectrometry, and receiver operating characteristic curves were constructed to show the diagnostic ability of the signature over time.

Results

Untargeted metabolomic analysis r

SUBMITTER: Laiakis EC 

PROVIDER: S-EPMC6815709 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature