17β-Estradiol nongenomically induces vascular endothelial H₂S release by promoting phosphorylation of cystathionine γ-lyase.

ABSTRACT: Estrogen exerts its cardiovascular protective role at least in part by regulating endothelial hydrogen sulfide (H₂S) release, but the underlying mechanisms remain to be fully elucidated. Estrogen exerts genomic effects, i.e. those involving direct binding of the estrogen receptor (ER) to gene promoters in the nucleus, and nongenomic effects, mediated by interactions of the ER with other proteins. Here, using human umbilical vein endothelial cells (HUVECs), immunological detection, MS-based analyses, and cGMP and H₂S assays, we show that 17β-estradiol (E2) rapidly enhances endothelial H₂S release in a nongenomic manner. We found that E2 induces phosphorylation of cystathionine γ-lyase (CSE), the key enzyme in vascular endothelial H₂S genera

SUBMITTER: Xu X 

PROVIDER: S-EPMC6816087 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature