Matrix Mechanics Influence Fibroblast-Myofibroblast Transition by Directing the Localization of Histone Deacetylase 4.

ABSTRACT:

Introduction

The propagation of mechanochemical signals from the extracellular matrix to the cell nucleus has emerged as a central feature in regulating cellular differentiation and de-differentiation. This process of outside-in signaling and the associated mechanotransduction pathways have been well described in numerous developmental and pathological contexts. However, it remains less clear how mechanotransduction influences the activity of chromatin modifying enzymes that direct gene expression programs.

Objectives

The primary objective of this study was to explore how matrix mechanics and geometric confinement influence histone deacetylase (HDAC) activity in fibroblast culture.

Methods

Polyacrylamide hydrogels were formed and functionalized with fibronectin patter

SUBMITTER: Li Y 

PROVIDER: S-EPMC6816600 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature