Dynamic Tracking of Osteoblastic Cell Traction Force during Guided Migration.

ABSTRACT:

Introduction

Continuous development of cell traction force can regulate cell migration on various extracellular matrixes in vivo. However, the topographical effect on traction force is still not fully understood.

Methods

Micropost sensors with parallel guiding gratings were fabricated in polydimethylsiloxane to track the cell traction force during topographical guidance in real time. The force distributions along MC3T3-E1 mouse osteoblasts were captured every minute. The traction force in the leading, middle, and trailing regions was monitored during forward and reversed cell migration.

Results

The traction force showed periodic changes during cell migration when the cell changed from elongated to contracted shape. For cell migration without guiding pattern, th

SUBMITTER: Hui J 

PROVIDER: S-EPMC6816708 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature