Cell Sequence and Mitosis Affect Fibroblast Directional Decision-Making During Chemotaxis in Microfluidic Mazes.

ABSTRACT:

Introduction

Directed fibroblast migration is central to highly proliferative processes in regenerative medicine and developmental biology. However, the mechanisms by which single fibroblasts affect each other's directional decisions, while chemotaxing in microscopic pores, are not well understood.

Methods

We explored effects of cell sequence and mitosis on fibroblast platelet-derived growth factor-BB (PDGF-BB)-induced migration in microfluidic mazes with two possible through paths: short and long. Additionally, image-based modeling of the chemoattractant's diffusion, consumption and decay, was used to explain the experimental observations.

Results

It both cases, the cells displayed behavior that is contradictory to expectation based on the global chemoattracta

SUBMITTER: Pham QL 

PROVIDER: S-EPMC6816736 | biostudies-literature | 2018 Dec

REPOSITORIES: biostudies-literature