Molecular prenatal diagnosis of alpha and beta thalassemia in pregnant Hakka women in southern China.

ABSTRACT:

Background

To date, there has been no systematic study of DNA-based prenatal diagnosis of thalassemia in pregnant Hakka women in southern China.

Methods

A total of 279 pregnant Hakka women with confirmed cases of thalassemia who had been treated at the Meizhou People's Hospital in China's Guangdong Province from January 2014 to December 2016 were here enrolled. Genomic DNA was extracted from peripheral blood of couples and villus, amniotic fluid, or fetal cord blood. DNA-based diagnosis was performed on the tissues of fetuses whose parents had tested positive for α- and β-globin gene mutations were found using polymerase chain reaction (PCR) and flow-through hybridization technique. Follow-up visits were performed 6 months after the fetuses were born. Prenatal diagnosis was

SUBMITTER: Zhao P 

PROVIDER: S-EPMC6816879 | biostudies-literature | 2018 Mar

REPOSITORIES: biostudies-literature