Protein Z and Endothelin-1 genetic polymorphisms in pediatric Egyptian sickle cell disease patients.

ABSTRACT:

Background

Sickle cell disease (SCD) is a monogenic disease associated with multisystem morbidity. Vasculopathy caused by delicate imbalance between coagulation and endothelial systems plays a pivotal role in disease course. As Protein Z and Endothelin-1 genetic polymorphisms may increase the thrombotic risk, the aim of the current work was to verify the possible impact of Protein Z (PROZ G79A) and Endothelin-1 (EDN1 G5665T) polymorphisms on the clinic-laboratory features of the SCD in a cohort of Egyptian pediatric patients.

Methods

Genotyping of Protein Z G79A and Endothelin-1 G5665T was carried out by polymerase chain reaction-restricted fragment length polymorphism (PCR-RFLP) assay for 100 SCD patients and 100 controls.

Results

Protein -Z G79A polymorphism was not

SUBMITTER: Khorshied MM 

PROVIDER: S-EPMC6816928 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature