High accuracy of proximity extension assay technology for the quantification of plasma brain natriuretic peptide.

ABSTRACT:

Background

Novel multiplex assays allow the simultaneous identification of a large number of plasma proteins. While these new technologies have been shown to be highly sensitive and accurate for the identification of plasma proteins, the use of this technology to quantify those proteins has not been properly investigated. In this pilot study, we tested the accuracy of the proximity extension assay (PEA) for the quantification of the cardiac biomarker brain natriuretic peptide (BNP) compared to a standard clinically approved method.

Methods

Concentrations of BNP were assessed in 120 plasma samples from 30 patients with PEA and compared to chemiluminescent microparticle immunoassay (CMIA). Venous blood samples were collected from in tubes containing ethylenediaminetetraacetic

SUBMITTER: Arrigo M 

PROVIDER: S-EPMC6817038 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature