Evaluation of the new restandardized Abbott Architect 25-OH Vitamin D assay in vitamin D-insufficient and vitamin D-supplemented individuals.

ABSTRACT:

Background

Recently, Abbott Diagnostics has restandardized the Architect 25(OH)D assay against the NIST SRM 2972. We have evaluated the analytical and clinical performance of the restandardized Architect 25(OH)D assay and compared its performance with a NIST-traceable liquid chromatography-tandem mass spectrometry (LC-MS/MS) method and the Roche total 25(OH)D assay in vitamin D-insufficient individuals before and after vitamin D₃ supplementation.

Methods

Frozen serum samples were obtained from 88 healthy subjects with self-perceived fatigue and vitamin D-insufficiency <50 nmol L^-1 who were randomized to receive a single 100 000 IU dose of vitamin D₃ (n = 48) or placebo (n = 40). Total 25(OH)D concentrations were measured before and 4 weeks af

SUBMITTER: Annema W 

PROVIDER: S-EPMC6817053 | biostudies-literature | 2018 May

REPOSITORIES: biostudies-literature