An improved reference method for serum cations measurement by ion chromatography.

ABSTRACT:

Background

To improve the accuracy of the routine methods in laboratory medicine, ion chromatography with a simple sample treatment procedure, which can completely remove the proteins and/or organics in human serum, has been developed for the determination of serum cations.

Methods

Chromatographic conditions for the separate and simultaneous determination of K, Na, Ca, and Mg were investigated. Furthermore, various factors influencing the mineralization of human serum, such as the selection and amount of oxidant, were also examined systematically and optimized.

Results

The optimized experimental conditions are as follows: 1.0 mL of serum specimen digested with 2 mL nitric acid (120°C) followed by 2 mL hydrogen peroxide (80°C). The specimens were then redissolved and d

SUBMITTER: Zou J 

PROVIDER: S-EPMC6817070 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature