Detecting the polymorphism of TERF1 gene by an improved PCR-RFLP method.

ABSTRACT: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) is a common and mature method of detecting the single nucleotide polymorphism (SNP). But, for the polymorphism site rs3863242 of telomeric repeat binding factor 1(TERF1) gene, there is no appropriate restriction enzyme to recognize it, which limits the research between the variants of rs3863242 and human diseases. The reverse primer was designed based on turning the 3rd base T into the mismatch base G. After PCR amplification, a new restriction enzyme site was introduced into the TERF1 gene amplification products. Two hundred forty samples from Chinese Han individuals were genotyped to evaluate this method. A new restriction enzyme site for CviQI was introduced into the PCR products. The genotype frequencies of 2

SUBMITTER: Wang P 

PROVIDER: S-EPMC6817086 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature