Characteristic and influencing factors of Taqman genotyping calling error.

ABSTRACT:

Background

Taqman fluorescent probe was frequently applied in single nucleotide polymorphism (SNP) genotyping. However, the characteristic of calling error and the influencing factors remain unclear.

Method

Calling errors of Taqman genotyping was evaluated systematically based on Mendelian inheritance. Twenty-two SNPs were genotyped by Taqman probe for 419 pedigrees. Mendelian genetic errors were counted for every SNP and pedigree. Cluster analysis was applied to investigate the compatibility between Taqman probes and DNA sample.

Results

On one hand, errors were found for all the SNPs. The error number ranged from 4 to 33 with median of 10.5. On the other hand, Mendelian genetic errors showed features of both randomness and cluster. Half of the pedigrees containing er

SUBMITTER: Yu S 

PROVIDER: S-EPMC6817118 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature