MiR-10a-5p is increased in atopic dermatitis and has capacity to inhibit keratinocyte proliferation.

ABSTRACT:

Background

miR-10a-5p has been shown to regulate cancer cell proliferation and invasiveness and endothelial cell inflammatory responses. The function of miR-10a-5p in the skin has not been previously studied. The aim of the current study was to examine miR-10a-5p expression, regulation, and function in keratinocytes (KCs) in association with atopic dermatitis (AD).

Methods

The expression of miR-10a-5p and its target genes was analyzed using RT-qPCR, mRNA array analysis, in situ hybridization, and immunofluorescence. The transfection of miRNA mimics, cell cycle distribution analysis, and luciferase assays was used to study miR-10a-5p functions in human primary KCs.

Results

miR-10a-5p was found to be upregulated in lesional skin from patients with AD and in proliferatin

SUBMITTER: Vaher H 

PROVIDER: S-EPMC6817370 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature