Conformational flexibility of adenine riboswitch aptamer in apo and bound states using NMR and an X-ray free electron laser.

ABSTRACT: Riboswitches are structured cis-regulators mainly found in the untranslated regions of messenger RNA. The aptamer domain of a riboswitch serves as a sensor for its ligand, the binding of which triggers conformational changes that regulate the behavior of its expression platform. As a model system for understanding riboswitch structures and functions, the add adenine riboswitch has been studied extensively. However, there is a need for further investigation of the conformational dynamics of the aptamer in light of the recent real-time crystallographic study at room temperature (RT) using an X-ray free electron laser (XFEL) and femtosecond X-ray crystallography (SFX). Herein, we investigate the conformational motions of the add adenine riboswitch aptamer domain, in the presence or absence of

SUBMITTER: Ding J 

PROVIDER: S-EPMC6817744 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature