Identification of novel targets for host-directed therapeutics against intracellular Staphylococcus aureus.

ABSTRACT: During patient colonization, Staphylococcus aureus is able to invade and proliferate within human cells to evade the immune system and last resort drugs such as vancomycin. Hijacking specific host molecular factors and/or pathways is necessary for pathogens to successfully establish an intracellular infection. In this study, we employed an unbiased shRNA screening coupled with ultra-fast sequencing to screen 16,000 human genes during S. aureus infection and we identified several host genes important for this intracellular pathogen. In addition, we interrogated our screening results to find novel host-targeted therapeutics against intracellular S. aureus. We found that silencing the human gene TRAM2 resulted in a significant reduction of intracellular bacterial load while host cell viabilit

SUBMITTER: Bravo-Santano N 

PROVIDER: S-EPMC6817851 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature