Biochemical and Structural Insights Concerning Triclosan Resistance in a Novel YX₇K Type Enoyl-Acyl Carrier Protein Reductase from Soil Metagenome.

ABSTRACT: Enoyl-acyl carrier protein reductase (ENR) catalyzes the last reduction step in the bacterial type II fatty acid biosynthesis cycle. ENRs include FabI, FabL, FabL2, FabK, and FabV. Previously, we reported a unique triclosan (TCL) resistant ENR homolog that was predominant in obligate intracellular pathogenic bacteria and Apicomplexa. Herein, we report the biochemical and structural basis of TCL resistance in this novel ENR. The purified protein revealed NADH-dependent ENR activity and shared similarity to prototypic FabI. Thus, this metagenome-derived ENR was designated FabI2. Unlike other prototypic bacterial ENRs with the YX₆K type catalytic domain, FabI2 possessed a unique YX₇K type catalytic domain. Computational modeling followed by site-directed mutagenesis reve

SUBMITTER: Khan R 

PROVIDER: S-EPMC6817880 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature