Determination of human γδ T cell-mediated cytotoxicity using a non-radioactive assay system.

ABSTRACT: The adoptive transfer of immune effector cells, such as CD8⁺ killer αβ T cells, γδ T cells, NK (natural killer) cells, and genetically-modified T cells, has been receiving increasing attention. It is essential to determine cellular cytotoxicity so as to monitor the function and quality of ex vivo-expanded immune effector cells before infusion. The most common method is the [⁵¹Cr]-sodium chromate release assay. It is, however, preferable to avoid the use of radioactive materials in clinical laboratories. In order to establish a non-radioactive alternative to the standard radioactive assay, we previously synthesized a chelate-forming prodrug (BM-HT) and demonstrated that a combination of BM-HT and europium (Eu³⁺) was useful to determine NK cell-mediated cytot

SUBMITTER: Tagod MSO 

PROVIDER: S-EPMC6817948 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature