Using BEAN-counter to quantify genetic interactions from multiplexed barcode sequencing experiments.

ABSTRACT: The construction of genome-wide mutant collections has enabled high-throughput, high-dimensional quantitative characterization of gene and chemical function, particularly via genetic and chemical-genetic interaction experiments. As the throughput of such experiments increases with improvements in sequencing technology and sample multiplexing, appropriate tools must be developed to handle the large volume of data produced. Here, we describe how to apply our approach to high-throughput, fitness-based profiling of pooled mutant yeast collections using the BEAN-counter software pipeline (Barcoded Experiment Analysis for Next-generation sequencing) for analysis. The software has also successfully processed data from Schizosaccharomyces pombe, Escherichia coli, and Zymomonas mobilis mutant colle

SUBMITTER: Simpkins SW 

PROVIDER: S-EPMC6818255 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature