Diversified Application of Barcoded PLATO (PLATO-BC) Platform for Identification of Protein Interactions.

ABSTRACT: Proteins usually associate with other molecules physically to execute their functions. Identifying these interactions is important for the functional analysis of proteins. Previously, we reported the parallel analysis of translated ORFs (PLATO) to couple ribosome display of full-length ORFs with affinity enrichment of mRNA/protein/ribosome complexes for the "bait" molecules, followed by the deep sequencing analysis of mRNA. However, the sample processing, from extraction of precipitated mRNA to generation of DNA libraries, includes numerous steps, which is tedious and may cause the loss of materials. Barcoded PLATO (PLATO-BC), an improved platform was further developed to test its application for protein interaction discovery. In this report, we tested the antisera-antigen interaction usin

SUBMITTER: Kong W 

PROVIDER: S-EPMC6818353 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature