Development and validation of a rapid method for genotyping three P-selectin gene polymorphisms based on high resolution melting analysis.

ABSTRACT:

Background

High resolution melting (HRM) analysis is one of the newer, reliable, and sensitive genotyping techniques, which offers considerable time and cost savings. P-selectin is an adhesion molecule that has a role in the initial phases of leukocyte adhesion to stimulated platelets and endothelial cells in inflammation. Multiple polymorphisms in P-selectin gene (SELP) that affect the protein sequence have been described. The aim of this study was to design, optimize, and validate a simple and rapid in-house HRM-based method for genotyping the NM_003005.3:c.992G>A (c.992G>A), NM_003005.3:c.1918G>T (c.1918G>T), and NM_003005.3:c.2266A>C (c.2266A>C) SELP polymorphisms.

Methods

Initial genotyping of three SELP polymorphisms was performed by applying polymerase chain reaction

SUBMITTER: Ceri A 

PROVIDER: S-EPMC6818544 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature