A low cost and input tailing method of quality control on multiple annealing, and looping-based amplification cycles-based whole-genome amplification products.

ABSTRACT:

Background

Single-cell whole-genome sequencing provides novel insights into the nature of genetic heterogeneity in normal and diseased cells. However, amplification of formalin-fixed tissues with low cell numbers is still problematic and multiple annealing, and looping-based amplification cycles (MALBAC) is a commonly used whole-genome amplification (WGA) method with low cell numbers.

Methods

We developed a low-input tailing method to evaluate the MALBAC-based WGA from sub-nanogram or less quantities of input DNA. The tailing method uses 2100 BioAnalyzer to evaluate the size distribution of MALBAC products, and comparing the tailing with 10380 bp.

Results

Compared with a 22 loci qPCR panel, the tailing method provided a similar WGA evaluation efficiency in 13 samples

SUBMITTER: Chen C 

PROVIDER: S-EPMC6818575 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature