M⁶A RNA Methylation Maintains Hematopoietic Stem Cell Identity and Symmetric Commitment.

ABSTRACT: Stem cells balance cellular fates through asymmetric and symmetric divisions in order to self-renew or to generate downstream progenitors. Symmetric commitment divisions in stem cells are required for rapid regeneration during tissue damage and stress. The control of symmetric commitment remains poorly defined. Using single-cell RNA sequencing (scRNA-seq) in combination with transcriptomic profiling of HSPCs (hematopoietic stem and progenitor cells) from control and m⁶A methyltransferase Mettl3 conditional knockout mice, we found that m⁶A-deficient hematopoietic stem cells (HSCs) fail to symmetrically differentiate. Dividing HSCs are expanded and are blocked in an intermediate state that molecularly and functionally resembles multipotent progenitors. Mechanistically,

SUBMITTER: Cheng Y 

PROVIDER: S-EPMC6818972 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature