CCR5 receptor antagonism inhibits hepatitis C virus (HCV) replication in vitro.

ABSTRACT:

Background and aim

The hepatitis C virus (HCV) is a single-strand RNA virus that infects millions of people worldwide. Recent advances in therapy have led to viral cure using two- and three- drug combinations of direct acting inhibitors of viral replication. CCR5 is a chemokine receptor that is expressed on hepatocytes and represents a key co-receptor for HIV. We evaluated the effect of CCR5 blockade or knockdown on HCV replication in Huh7.5JFH1 cells.

Methods

Cells were exposed to varying concentrations of maraviroc (CCR5 inhibitor), cenicriviroc (CCR2/CCR5 inhibitor), sofosbuvir (nucleotide polymerase inhibitor), or raltegravir (HIV integrase inhibitor).

Results

HCV RNA was detected utilizing two qualitative strand-specific RT-PCR assays. HCV core antigen and NS3 pr

SUBMITTER: Blackard JT 

PROVIDER: S-EPMC6818973 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature