Plasma deconvolution identifies broadly neutralizing antibodies associated with hepatitis C virus clearance.

ABSTRACT: A vaccine for hepatitis C virus (HCV) is urgently needed. Development of broadly-neutralizing plasma antibodies during acute infection is associated with HCV clearance, but the viral epitopes of these plasma antibodies are unknown. Identification of these epitopes could define the specificity and function of neutralizing antibodies (NAbs) that should be induced by a vaccine. Here, we present development and application of a high-throughput method that deconvolutes polyclonal anti-HCV NAbs in plasma, delineating the epitope specificities of anti-HCV NAbs in acute infection plasma of forty-four humans with subsequent clearance or persistence of HCV. Remarkably, we identified multiple broadly neutralizing antibody (bNAb) combinations that were associated with greater plasma neutralizing bread

SUBMITTER: Kinchen VJ 

PROVIDER: S-EPMC6819096 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature