AdpA_lin, a Pleiotropic Transcriptional Regulator, Is Involved in the Cascade Regulation of Lincomycin Biosynthesis in Streptomyces lincolnensis.

ABSTRACT: Lincomycin is one of the most important antibiotics in clinical practice. To further understand the regulatory mechanism on lincomycin biosynthesis, we investigated a pleiotropic transcriptional regulator AdpA_lin in the lincomycin producer Streptomyces lincolnensis NRRL 2936. Deletion of adpA _lin (which generated ΔadpA _lin ) interrupted lincomycin biosynthesis and impaired the morphological differentiation. We also found that putative AdpA binding sites were unusually scattered in the promoters of all the 8 putative operons in the lincomycin biosynthetic gene cluster (BGC). In ΔadpA _lin , transcript levels of structural genes in 8 putative operons were decreased with varying degrees, and electropho

SUBMITTER: Kang Y 

PROVIDER: S-EPMC6819324 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature