Characterization and pathogenicity of Vero cell-attenuated porcine epidemic diarrhea virus CT strain.

ABSTRACT:

Background

Porcine epidemic diarrhea virus (PEDV) has caused enormous economic losses to the global pig industry. Currently available PEDV vaccine strains have limited protective effects against PEDV variant strains.

Methods

In this study, the highly virulent epidemic virus strain CT was serially passaged in Vero cells for up to 120 generations (P120). Characterization of the different passages revealed that compared with P10 and P64, P120 had a higher viral titer and more obvious cytopathic effects, thereby demonstrating better cell adaptability.

Results

Pathogenicity experiments using P120 in piglets revealed significant reductions in clinical symptoms, histopathological lesions, and intestinal PEDV antigen distribution; the piglet survival rate in the P120 group wa

SUBMITTER: Wu Y 

PROVIDER: S-EPMC6819350 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature