3D-tips: user-friendly mesh barrier pipette tips for 3D-spheroid culture.

ABSTRACT:

Background

Three dimensional (3D) cell cultures have been an area of increasing interest and relevance across several research fields including drug discovery, developmental biology and stem cell-based therapies. However, handling 3D structures can be difficult. In particular, the replacement of liquid media and reagents in which liquid is removed using pipettes is difficult to perform as the 3D spheroids can be easily aspirated into the pipette tip.

Results

We have developed the 3D-tip, a novel tool that facilitates media change and washing procedures of 3D-spheroid cultures. The 3D-tip contains a mesh with 40-μm pores allowing the aspiration of liquids including media, drugs, buffers and reagents, with the mesh acting as a barrier preventing the spheroids being aspirated i

SUBMITTER: Chen M 

PROVIDER: S-EPMC6819412 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature