Multiplex PCR-based titration (MPBT) assay for determination of infectious titers of the three Sabin strains of live poliovirus vaccine.

ABSTRACT:

Background

Conventional assays to titrate polioviruses usually test serial dilutions inoculated into replicate cell cultures to determine a 50% cytopathic endpoint, a process that is both time-consuming and laborious. Such a method is still used to measure potency of live Oral Poliovirus Vaccine during vaccine development and production and in some clinical trials. However, the conventional method is not suited to identify and titrate virus in the large numbers of fecal samples generated during clinical trials. Determining titers of each of the three Sabin strains co-existing in Oral Poliovirus Vaccine presents an additional challenge.

Results

A new assay using quantitative multiplex polymerase chain reaction as an endpoint instead of cytopathic effect was developed to overc

SUBMITTER: Manukyan H 

PROVIDER: S-EPMC6819588 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature