Standardizing Automated DNA Assembly: Best Practices, Metrics, and Protocols Using Robots.

ABSTRACT: The advancement of synthetic biology requires the ability to create new DNA sequences to produce unique behaviors in biological systems. Automation is increasingly employed to carry out well-established assembly methods of DNA fragments in a multiplexed, high-throughput fashion, allowing many different configurations to be tested simultaneously. However, metrics are required to determine when automation is warranted based on factors such as assembly methodology, protocol details, and number of samples. The goal of our synthetic biology automation work is to develop and test protocols, hardware, and software to investigate and optimize DNA assembly through quantifiable metrics. We performed a parameter analysis of DNA assembly to develop a standardized, highly efficient, and reproducible Mo

SUBMITTER: Walsh DI 

PROVIDER: S-EPMC6819997 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature