Comparison of Staphylococcus aureus surface protein extraction methods and immunogenicity.

ABSTRACT: Staphylococcus aureus is the major contagious bovine mastitis pathogen and has no effective vaccine. Strain variation and limited knowledge of common immunogenic antigen/s are among major constraints for developing effective vaccines. S. aureus cell surface proteins that are exposed to the host immune system constitute good vaccine candidates. The objective of this study was to compare two novel S. aureus surface protein extraction methods with biotinylation method and evaluate immune-reactivity of extracted proteins. Surface proteins were extracted from nine genetically distinct S. aureus strains from cases of bovine mastitis. After extraction, bacterial cell integrity was examined by Gram staining and electron microscopy to determine if extraction methods caused damage to cells that may

SUBMITTER: Abdi RD 

PROVIDER: S-EPMC6820086 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature