SIRT3-mediated deacetylation of PRDX3 alleviates mitochondrial oxidative damage and apoptosis induced by intestinal ischemia/reperfusion injury.

ABSTRACT:

Background

Hydrogen peroxide (H₂O₂)-induced mitochondrial oxidative damage is critical to intestinal ischemia/reperfusion (I/R) injury, and PRDX3 is an efficient H₂O₂ scavenger that protects cells from mitochondrial oxidative damage and apoptosis. However, the function of PRDX3 in intestinal I/R injury is unclear. The aim of this study was to investigate the precise mechanism underlying the involvement of PRDX3 in intestinal I/R injury.

Methods

An intestinal I/R model was established in mice with superior mesenteric artery occlusion, and Caco-2 cells were subjected to hypoxia/reoxygenation (H/R) for the in vivo simulation of I/R.

Results

PRDX3 expression was decreased during intestinal I/R injury, and PRDX3 overexpression si

SUBMITTER: Wang Z 

PROVIDER: S-EPMC6820261 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature