Significance of transient receptor potential vanilloid 4 and aquaporin 5 co-expression in the rat uterus at term.

ABSTRACT:

Aims

Aquaporins (AQPs) are channel proteins that facilitate the rapid passive movement of water. In our studies it was proved that the decreased AQP5 expression is followed by the increase of uterine contractility. The transient receptor potential vanilloid 4 (TRPV4) is a calcium channel, which is activated in response to osmotic changes. Our aim was to determine the possible role of AQP5 in this osmotic regulation of TRPV4, thus in pregnant uterine contraction.

Main methods

We used RT-PCR and Western blot techniques for the detection of the TRPV4 expression during pregnancy in rat uterus. The localization of AQP5 and TRPV4 was determined by immunohistochemical studies. The role of TRPV4 in uterus contraction was investigated in an isolated organ bath system. In vitro

SUBMITTER: Ducza E 

PROVIDER: S-EPMC6820280 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature