Effects of codon optimization on coagulation factor IX translation and structure: Implications for protein and gene therapies.

ABSTRACT: Synonymous codons occur with different frequencies in different organisms, a phenomenon termed codon usage bias. Codon optimization, a common term for a variety of approaches used widely by the biopharmaceutical industry, involves synonymous substitutions to increase protein expression. It had long been presumed that synonymous variants, which, by definition, do not alter the primary amino acid sequence, have no effect on protein structure and function. However, a critical mass of reports suggests that synonymous codon variations may impact protein conformation. To investigate the impact of synonymous codons usage on protein expression and function, we designed an optimized coagulation factor IX (FIX) variant and used multiple methods to compare its properties to the wild-type FIX upon exp

SUBMITTER: Alexaki A 

PROVIDER: S-EPMC6820528 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature