MRNA and miRNA Transcriptome Profiling of Granulosa and Theca Layers From Geese Ovarian Follicles Reveals the Crucial Pathways and Interaction Networks for Regulation of Follicle Selection.

ABSTRACT: Follicle development is characterized by the recruitment, growth, selection, and dominance of follicles, and follicle selection determines the lifetime reproductive performance. However, in birds, the molecular mechanisms underlying follicle selection still remain elusive. This study analyzed genome-wide changes in the mRNA and miRNA expression profiles in both the granulosa and theca layers of geese ovarian follicles before selection (4-6- and 8-10-mm follicles) and after selection (F5). The sequencing results showed that a higher number of both differentially expressed (DE) mRNAs and DE miRNAs were identified between 8-10-mm and F5 follicles compared with those between the 4-6- and 8-10-mm follicles, especially in the granulosa layer. Moreover, a Short Time-series Expression Miner analys

SUBMITTER: Li Q 

PROVIDER: S-EPMC6820619 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature