A 96-multiplex capillary electrophoresis screening platform for product based evolution of P450 BM3.

ABSTRACT: The main challenge that prevents a broader application of directed enzyme evolution is the lack of high-throughput screening systems with universal product analytics. Most directed evolution campaigns employ screening systems based on colorimetric or fluorogenic surrogate substrates or universal quantification methods such as nuclear magnetic resonance spectroscopy or mass spectrometry, which have not been advanced to achieve a high-throughput. Capillary electrophoresis with a universal UV-based product detection is a promising analytical tool to quantify product formation. Usage of a multiplex system allows the simultaneous measurement with 96 capillaries. A 96-multiplexed capillary electrophoresis (MP-CE) enables a throughput that is comparable to traditional direct evolution campaigns e

SUBMITTER: Gartner A 

PROVIDER: S-EPMC6820799 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature