In Vivo Function of the Lipid Raft Protein Flotillin-1 during CD8⁺ T Cell-Mediated Host Surveillance.

ABSTRACT: Flotillin-1 (Flot1) is an evolutionary conserved, ubiquitously expressed lipid raft-associated scaffolding protein. Migration of Flot1-deficient neutrophils is impaired because of a decrease in myosin II-mediated contractility. Flot1 also accumulates in the uropod of polarized T cells, suggesting an analogous role in T cell migration. In this study, we analyzed morphology and migration parameters of murine wild-type and Flot1^-/- CD8⁺ T cells using in vitro assays and intravital two-photon microscopy of lymphoid and nonlymphoid tissues. Flot1^-/- CD8⁺ T cells displayed significant alterations in cell shape and motility parameters in vivo but showed comparable homing to lymphoid organs and intact in vitro migration to chemokines. Furthermore, their

SUBMITTER: Ficht X 

PROVIDER: S-EPMC6820809 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature