Polymer-Salt Aqueous Two-Phase System (ATPS) Micro-Droplets for Cell Encapsulation.

ABSTRACT: Biosample encapsulation is a critical step in a wide range of biomedical and bioengineering applications. Aqueous two-phase system (ATPS) droplets have been recently introduced and showed a great promise to the biological separation and encapsulation due to their excellent biocompatibility. This study shows for the first time the passive generation of salt-based ATPS microdroplets and their biocompatibility test. We used two ATPS including polymer/polymer (polyethylene glycol (PEG)/dextran (DEX)) and polymer/salt (PEG/Magnesium sulfate) for droplet generation in a flow-focusing geometry. Droplet morphologies and monodispersity in both systems are studied. The PEG/salt system showed an excellent capability of uniform droplet formation with a wide range of sizes (20-60 μm) which makes it a s

SUBMITTER: Mastiani M 

PROVIDER: S-EPMC6820865 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature