Inflammatory cytokines and organ dysfunction associate with the aberrant DNA methylome of monocytes in sepsis.

ABSTRACT:

Background

Sepsis, a life-threatening organ dysfunction caused by a dysregulated systemic immune response to infection, associates with reduced responsiveness to subsequent infections. How such tolerance is acquired is not well understood but is known to involve epigenetic and transcriptional dysregulation.

Methods

Bead arrays were used to compare global DNA methylation changes in patients with sepsis, non-infectious systemic inflammatory response syndrome, and healthy controls. Bioinformatic analyses were performed to dissect functional reprogramming and signaling pathways related to the acquisition of these specific DNA methylation alterations. Finally, in vitro experiments using human monocytes were performed to test the induction of similar DNA methylation reprogramming.

SUBMITTER: Lorente-Sorolla C 

PROVIDER: S-EPMC6820973 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature