PacBio single-molecule long-read sequencing shed new light on the complexity of the Carex breviculmis transcriptome.

ABSTRACT: BACKGROUND:Carex L., a grass genus commonly known as sedges, is distributed worldwide and contributes constructively to turf management, forage production, and ecological conservation. The development of next-generation sequencing (NGS) technologies has considerably improved our understanding of transcriptome complexity of Carex L. and provided a valuable genetic reference. However, the current transcriptome is not satisfactory mainly because of the enormous difficulty in obtaining full-length transcripts. RESULTS:In this study, we employed PacBio single-molecule long-read sequencing (SMRT) technology for whole-transcriptome profiling in Carex breviculmis. We generated 60,353 high-confidence non-redundant transcripts with an average length of 2302-bp. A total of 3588 alternative splicing e

SUBMITTER: Teng K 

PROVIDER: S-EPMC6821003 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature