Estrogen-induced compensatory mechanisms protect IL-10-deficient mice from developing EAE.

ABSTRACT:

Background

IL-10 knockout (KO) mice are protected from experimental autoimmune encephalomyelitis (EAE) with low-dose estrogen (E2) treatment similar to wild-type (WT) mice. Previous studies have demonstrated a decrease in tumor necrosis factor in all E2-treated groups, which led to the protection of the mice.

Methods

This study used IL-10 KO mice and WT mice treated either with E2 or sham pellets 7 days prior to induction of EAE. Mice were observed for 21 days post-immunization. The spleen, inguinal lymph nodes, and brain were evaluated by flow cytometry. Spinal cords were evaluated using a cytokine/chemokine array, RT-PCR, and histology.

Results

This study demonstrates that E2 treatment induced three heightened regulatory mechanisms that potentially protect IL-10 KO

SUBMITTER: Seifert HA 

PROVIDER: S-EPMC6821034 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature