Genome Assembly of the A-Group Wolbachia in Nasonia oneida Using Linked-Reads Technology.

ABSTRACT: Wolbachia are obligate intracellular bacteria which commonly infect various nematode and arthropod species. Genome sequences have been generated from arthropod samples following enrichment for the intracellular bacteria, and genomes have also been assembled from arthropod whole-genome sequencing projects. However, these methods remain challenging for infections that occur at low titers in hosts. Here we report the first Wolbachia genome assembled from host sequences using 10× Genomics linked-reads technology. The high read depth attainable by this method allows for recovery of intracellular bacteria that are at low concentrations. Based on the depth differences (714× for the insect and 59× for the bacterium), we assembled the genome of a Wolbachia in the parasitoid jewel wasp species Nason

SUBMITTER: Wang X 

PROVIDER: S-EPMC6821208 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature