MyelinJ: an ImageJ macro for high throughput analysis of myelinating cultures.

ABSTRACT:

Summary

MyelinJ is a free user friendly ImageJ macro for high throughput analysis of fluorescent micrographs such as 2D-myelinating cultures and statistical analysis using R. MyelinJ can analyse single images or complex experiments with multiple conditions, where the ggpubr package in R is automatically used for statistical analysis and the production of publication quality graphs. The main outputs are percentage (%) neurite density and % myelination. % neurite density is calculated using the normalize local contrast algorithm, followed by thresholding, to adjust for differences in intensity. For % myelination the myelin sheaths are selected using the Frangi vesselness algorithm, in conjunction with a grey scale morphology filter and the removal of cell bodies using a high intensit

SUBMITTER: Whitehead MJ 

PROVIDER: S-EPMC6821319 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature