Single primer enrichment technology as a tool for massive genotyping: a benchmark on black poplar and maize.

ABSTRACT:

Background and aims

The advent of molecular breeding is advocated to improve the productivity and sustainability of second-generation bioenergy crops. Advanced molecular breeding in bioenergy crops relies on the ability to massively sample the genetic diversity. Genotyping-by-sequencing has become a widely adopted method for cost-effective genotyping. It basically requires no initial investment for design as compared with array-based platforms which have been shown to offer very robust assays. The latter, however, has the drawback of being limited to analyse only the genetic diversity accounted during selection of a set of polymorphisms and design of the assay. In contrast, genotyping-by-sequencing with random sampling of genomic loci via restriction enzymes or random priming has b

SUBMITTER: Scaglione D 

PROVIDER: S-EPMC6821380 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature