More than efficacy revealed by single-cell analysis of antiviral therapeutics.

ABSTRACT: Because many aspects of viral infection dynamics and inhibition are governed by stochastic processes, single-cell analysis should provide more information than approaches using population averaging. We have developed a microfluidic device composed of ~6000 wells, with each well containing a microstructure to capture single, infected cells replicating an enterovirus expressing a fluorescent reporter protein. We have used this system to characterize enterovirus inhibitors with distinct mechanisms of action. Single-cell analysis reveals that each class of inhibitor interferes with the viral infection cycle in a manner that can be distinguished by principal component analysis. Single-cell analysis of antiviral candidates not only reveals efficacy but also facilitates clustering of drugs with t

SUBMITTER: Liu W 

PROVIDER: S-EPMC6821460 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature