Marine Archaeon Methanosarcina acetivorans Enhances Polyphosphate Metabolism Under Persistent Cadmium Stress.

ABSTRACT: Phosphate metabolism was studied to determine whether polyphosphate (polyP) pools play a role in the enhanced resistance against Cd²⁺ and metal-removal capacity of Cd²⁺-preadapted (CdPA) Methanosarcina acetivorans. Polyphosphate kinase (PPK), exopolyphosphatase (PPX) and phosphate transporter transcript levels and their activities increased in CdPA cells compared to control (Cnt) cells. K⁺ inhibited recombinant Ma-PPK and activated Ma-PPX, whereas divalent cations activated both enzymes. Metal-binding polyP and thiol-containing molecule contents, Cd²⁺-removal, and biofilm synthesis were significantly higher in CdPA cells >Cnt cells plus a single addition of Cd²⁺>Cnt cells. Also, CdPA cells showed a higher number of cadmiu

SUBMITTER: Jasso-Chavez R 

PROVIDER: S-EPMC6821655 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature