Deciphering the Functioning of Microbial Communities: Shedding Light on the Critical Steps in Metaproteomics.

ABSTRACT: Unraveling the complex structure and functioning of microbial communities is essential to accurately predict the impact of perturbations and/or environmental changes. From all molecular tools available today to resolve the dynamics of microbial communities, metaproteomics stands out, allowing the establishment of phenotype-genotype linkages. Despite its rapid development, this technology has faced many technical challenges that still hamper its potential power. How to maximize the number of protein identification, improve quality of protein annotation, and provide reliable ecological interpretation are questions of immediate urgency. In our study, we used a robust metaproteomic workflow combining two protein fractionation approaches (gel-based versus gel-free) and four protein search datab

SUBMITTER: Geron A 

PROVIDER: S-EPMC6821674 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature