Characterisation of proguanylin expressing cells in the intestine - evidence for constitutive luminal secretion.

ABSTRACT: Guanylin, a peptide implicated in regulation of intestinal fluid secretion, is expressed in the mucosa, but the exact cellular origin remains controversial. In a new transgenic mouse model fluorescent reporter protein expression driven by the proguanylin promoter was observed throughout the small intestine and colon in goblet and Paneth(-like) cells and, except in duodenum, in mature enterocytes. In Ussing chamber experiments employing both human and mouse intestinal tissue, proguanylin was released predominantly in the luminal direction. Measurements of proguanylin expression and secretion in cell lines and organoids indicated that secretion is largely constitutive and requires ER to Golgi transport but was not acutely regulated by salt or other stimuli. Using a newly-developed proguanyli

SUBMITTER: Dye FS 

PROVIDER: S-EPMC6821700 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature