Uniform Expression and Relatively Small Position Effects Characterize Sister Transformants in Maize and Soybean.

ABSTRACT: Development of transgenic cell lines or organisms for industrial, agricultural, or medicinal applications involves inserting DNA into the target genome in a way that achieves efficacious transgene expression without a deleterious impact on fitness. The genomic insertion site is widely recognized as an important determinant of success. However, the effect of chromosomal location on transgene expression and fitness has not been systematically investigated in plants. Here we evaluate the importance of transgene insertion site in maize and soybean using both random and site-specific transgene integration. We have compared the relative contribution of genomic location on transgene expression levels with other factors, including cis-regulatory elements, neighboring transgenes, genetic background

SUBMITTER: Betts SD 

PROVIDER: S-EPMC6821721 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature